The Transgenic Mouse Core located in the School of Veterinary Medicine is a state-of-the-art fee-for-service facility that offers a full line of embryological manipulation services, focused on, but not limited to, murine model systems. The Transgenic and Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. (J.Cell Biol. Redirecting to /core/journals/journal-of-dairy-research/article/abs/expression-of-bovine-lactoglobulin-transgenic-mice 5C), while inserting a . To achieve these goals, the School of Veterinary Medicine has established the Transgenic Mouse Core. FOIA The tryptophan synthetase gene A series of mutants in E. coli has been used to examine the mutational specificity of over 80 purine base analogues. The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines. Kozmin SG, Schaaper RM, Shcherbakova PV, Kulikov VN, Noskov VN, Guetsova ML, Alenin VV, Rogozin IB, Makarova KS, Pavlov YI. The centralization of these technically demanding procedures in the Core laboratory results in enhanced efficiency and significant cost reduction for the DRC community. For more information about our services, charges for services or personnel please visit the official Transgenic & Chimeric Mouse Facility. 2010 Jul;33(3):549-57. doi: 10.1590/S1415-47572010005000060. They offer histological services, equipment, and technical expertise to assist in a variety of research projects. Mutat Res. The mission of the core is to offer a "turn-key service" for new animal models at PBRC that will allow for the controlled manipulation of gene expression (knock-in point mutations, knock-in of cre recombinase, knock-in of floxed transcriptional blockers, and knock-in of human cDNA, into homologous mouse genes etc) and facilitate investigators. Care for cats, dogs & other companion animals. In addition to these specialized services, the facility will work with individual laboratories to customize assays/experiments upon request. As the Associate Dean for Research Integration, I want to welcome . Philadelphia, PA 19104. University of Pennsylvania Perelman School of Medicine Transgenic and and re-derivation of live and cryopreserved lines. The injection rooms are fully equipped to carry out the entire procedure of making transgenic mice. TRANSGENIC ANIMALS used at Penn include but are not limited to insects, nematodes, fish, amphibians, and rodents. 4 purine analogues have been discovered that solely cause transversions. Email: kthompso@pennmedicine.upenn.edu, Tel: 215-573-3023; 215-746-6392 These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. Study and manipulation of male germ line stem cells has implications for control of fertility, large animal transgenesis and genetic preservation. In laymens terms, they are siblings born to the same surrogate mother, exhibiting different combinations of the same genetic mixture. 1 Through intramolecular recombination, cre has the ability to excise loxP-flanked (floxed) sequences from the genome, leaving a single loxP site in its place. The efficiency and extent of mutagenic activity of some new mutagens of base-analogue type. BUMC: Transgenic Mice Core . Penn Vet | Transgenic Mouse Core - University of Pennsylvania School of 2A6MAP, 2-amino-6-ethylaminopurine and 2,6-bismethylaminopurine stimulate only the AT----CG transversion while 2MAP additionally gives rise to AT----TA transversions. Care for cats, dogs & other companion animals. kpiotro@emory.edu. Bookshelf Penn Vet | Research Center - University of Pennsylvania School of We thank Jean Richa and the University of Pennsylvania Transgenic Core Facility and Ray Meade and the University of Pennsylvania Biomedical Imaging Core Facility for electron microscopy analysis and the Jefferson Kimmel Cancer Center Bioimaging Facility for confocal imaging. PMC Genotoxicity and mutagenicity of Echinodorus macrophyllus (chapu-de-couro) extracts. D. David C. Schultz, Ph. DMOZ - Science: Biology: Genetics: Eukaryotic: Animal: Mammal: Rodent Douglas Epstein, Ph.D., is the Facility Director. If you have a bachelor's degree in a non-nursing discipline . Department of Genomic Analysis Core - Abramson Cancer Center | Penn Medicine PDF TRANSGENIC ANIMALS NIH Guidelines for Research Involving - UPenn The construct was injected into FVB mouse oocytes by the University of Pennsylvania Transgenic and Chimeric Mouse Facility. The Core also oversees a cyropreservation facility for long-term storage of mouse embryos and sperm samples. Influence of sex, smoking and age on human hprt mutation frequencies and spectra. Would you like email updates of new search results? The SAIF combines state-of-the-art instrumentation and a nationally recognized staff to assist investigators with a wide range of imaging based experimental approaches. Contact David Schultz at dschultz@mail.med.upenn.edu for an initial consultation Define the project Determine if the facility has relevant expertise/technology to pursue the project Graduates from the School of Nursing have strong clinical skills and a passion to excel in the fields of nursing and health care. For CL 316,243 treatment, Ebf2 . It is proposed that a major mechanism for hydroxylaminopurine mutagenesis is due to the reaction of these compounds with the O6-position of guanine and the O4-position of thymine. A transgenic rescue of the AmelX / enamel using the full-length amelogenin gene (M180) was largely successful and restored enamel prism structure and properties (Fig. Magnetic resonance imaging (MRI) and spectroscopy (MRS), Optical imaging (including bioluminescence, fluorescence, and near-infrared imaging), Single photon emission computed tomography (SPECT). Please enable it to take advantage of the complete set of features! Cardiac Tissue-Restricted Deletion of Plakoglobin Results in What strain of mice are used as foster mothers? Department of Genetics PDF WHEN - Perelman School of Medicine at the University of Pennsylvania To apply for membership, please send a cover letter and CV to Dr. Wang. The facility is located on the basement level of the Clinical Research Building. Direct microinjection into the pronuclei of fertilized single-cell mouse embryos. For Es Cell derivation & gene targeting please contact Chris Lengner (lengner@vet.upenn.edu) & Stephanie Adams (asteph@vet.upenn.edu). Marion Dilley and David George Jones Endowed Chair in Animal Reproduction, Associate Professor, Department of Biomedical Sciences. Steven's animal research career began at University of Pennsylvania as an Animal Laboratory Technician. What strain are the donor embryos used for microinjection? About | Emory University | Atlanta GA It also allows for ease of sharing mouse strains with collaborators across the world. Phone: 215-573-2302, Stephanie Sterling, Lab Manager Association of Biomolecular Resource Facilities. Study of molecular mechanisms of mammalian sperm motility and testicular degeneration has implications for treatment of infertility. Privacy Policy | The Facility offers services including DNA pronuclear injection into fertilized oocytes (along with genotyping of transgenic founders), ES cell injection into blastocysts, cytoplasmic/pronuclear injections into fertilized oocytes of CRISPR-Cas9 mix (gRNA, Cas9RNA, ssDNA/dsDNA templates), embryo and sperm cryopreservation, in vitro fertilization, and re-derivation of live and cryopreserved lines. Kathleen Moosbrugger and Pei-Fu He are Research Specialists who assist in various aspects of the facilitys services. The amelogenin C-terminus is required for enamel development. - Europe PMC The last 4 analogues also cause transitions. The School of Nursing requires you to participate in an interview. Cambridge University Press & Assessment The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). M180 Amelogenin Processed by MMP20 is Sufficient for Decussating Murine Policies - Penn Genetics supported browser. Amelx and Mmp20 mutations cause amelogenesis imperfecta. . - CORE . Smilow Center for Translational Research Their will be a $25/yr storage fee for anyone wanting to store their sperm straws in house. The center provides a platform for intellectual interactions to facilitate research in reproductive biology across the school and the campus. An improved understanding of the basic processes governing germ cell and embryo development and of the biology of gametes (sperm and oocytes) and embryonic stem cells will enable us to improve reproductive efficiency, generate animal models of human and animal disease and help provide the knowledge base for regenerative medicine, as well as toward the treatment of infertility. The Transgenic Mouse Coreis equipped with state-of-the-art equipment and is supported by fully trained personnel. 2001 Jul 15;29(14):3099-107. doi: 10.1093/nar/29.14.3099. Transgenics -- Transgenic Mice - Edinformatics Perelman School of Medicine Generation of Cre Transgenic Mice with Postnatal RPE-Specific Ocular 8 purine base analogues have been found that induce the AT----CG transversion at the trp A88 site; and 2-amino-6-methylaminopurine (2A6MAP) stimulates by 124-fold, 2-amino-6-ethylaminopurine by 20-fold, 2-methylaminopurine (2MAP) by 9.4-fold, 2,6-bismethylaminopurine by 25-fold, 2AP by 230-fold, 2,6DAP by 15-fold, 2.6-diaminopurine riboside by 5-fold, and 2-hydroxylaminopurine by 11-fold. Federal government websites often end in .gov or .mil. Site Design: DART Web Team. The mice featured are three littermates generated by the injection of ES cells into mouse blastocysts. D. David C. Schultz, Ph. EBF2 promotes the recruitment of beige adipocytes in white adipose Penn is highly ranked nation-wide among schools of medicine in PHS-funded biomedical research. 3400 Civic Center Boulevard, 12th Floor The animal room provides housing and breeding space for the mice involved in the transgenic projects. Clinical Research Building 500 8600 Rockville Pike The transversion-causing purine analogues are derivatives of 2-aminopurine (2AP) and 2,6-diaminopurine (2,6DAP). A major objective of this research is to improve the health and productivity of domestic animals by genetic modification and to develop animal models for biomedical research. Phone: 215-573-2301, 3800 Spruce Street - Philadelphia, PA 19104, Richard King Mellon Professor of Reproductive Physiology, Research Professor of Obstetrics and Gynecology, Assistant Professor, Department of Biomedical Sciences, Associate Professor, CE, of Medicine and Reproduction, Associate Professor, Section of Reproduction and Behavior, New Bolton Center, Clinical Assistant Professor, Biomedical Sciences, School of Veterinary Medicine. Tel: 215-573-4810 Accounting, Department of (Wharton) Accounts Payable (University) Ackoff Center for Advancement of Systems Approaches (ACASA) Acquisition Services. Philadelphia, PA 19104 <!-- /* Font Definitions */ @font-face {font-family:"MS Mincho"; panose-1:2 2 6 9 4 2 5 8 3 4; mso-font-alt:" "; mso-font-charset:128; mso-generic-font-family:modern; mso-font-pitch:fixed; mso-font-signature:-536870145 1791491579 134217746 0 131231 0;} @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536870145 1107305727 0 0 415 0;} @font-face {font-family:Times; panose-1:0 0 5 0 0 0 0 2 0 0; mso-font-alt:Times; mso-font-charset:0; mso-generic-font-family:auto; mso-font-pitch:variable; mso-font-signature:-536870145 1342185562 0 0 415 0;} @font-face {font-family:Garamond; panose-1:2 2 4 4 3 3 1 1 8 3; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:647 2 0 0 159 0;} @font-face {font-family:Perpetua; panose-1:2 2 5 2 6 4 1 2 3 3; mso-font-charset:77; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:3 0 0 0 1 0;} @font-face {font-family:"\@MS Mincho"; panose-1:2 2 6 9 4 2 5 8 3 4; mso-font-charset:128; mso-generic-font-family:modern; mso-font-pitch:fixed; mso-font-signature:-536870145 1791491579 134217746 0 131231 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0in; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Garamond",serif; mso-fareast-font-family:"MS Mincho"; mso-fareast-theme-font:minor-fareast; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-family:"Garamond",serif; mso-ascii-font-family:Garamond; mso-fareast-font-family:"MS Mincho"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Garamond; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}size:8.5in 11.0in; margin:1.0in 1.0in 1.0in 1.0in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.WordSection1 {page:WordSection1;}. Free full text J Dent Res. University of Pennsylvania Transgenic and Chimeric Mouse Facility . Cre recombinase is a P1 bacteriophage protein that binds to a 34-bp-long target recognition site known as loxP. D. dschultz@mail.med.upenn.edu (215) 5739641 . PDF TRANSGENIC & GENE TARGETING MOUSE FACILITY Department of Biomedical The injection rooms are fully equipped to carry out the entire procedure of making transgenic mice. We do not charge you a service fee unless we produce a founder. For sperm cryopreservation we require 2 males per strain. The Molecular Pathology and Imaging Core is a resource for researchers at Penn, CHOP, and beyond. However there is a clear indication that such development is shifting towards 1 Volume 1, Number 1, 2006. the peripheries. PDF University of Pennsylvania Perelman School of Medicine High Throughput Jean Richa, Ph.D., is the Technical Director in charge of the production of transgenic mice. The Transgenic Mouse Coreat the Center (Technical director, N. Adrian Leu; Faculty oversight: P. Jeremy Wang MD/PhD and Christopher Lengner, PhD) provides an array of embryological services for the generation and preservation of genetically engineered transgenic and gene knockout mice. Go to: Abstract Amelogenin (AMELX) and matrix metalloproteinase-20 (MMP20) are essential for proper enamel development. These mice carry randomly inserted transgenes and/or site-specific alterations in the mouse genome of specific interest to Penn researchers. University of Pennsylvania Perelman School of Medicine High-Throughput Screening Core Sara Cherry, Ph. Scientists at the School of Veterinary Medicine performed pioneering studies in the development of transgenic techniques and were responsible for production of the first transgenic mice and farm animals. Mutagenicity of 2-aminopurine, 6-N-hydroxylaminopurine, and 2-amino-N6-hydroxyadenine in Neurospora crassa. Basic Life Sci. An official website of the United States government. The population aging and an increased life expectancy are widely recognized social changes. The .gov means its official. Various advanced producer services such as research and After you submit your application, you should contact the school directly for more information and to discuss previous academic work. 1978 Jan;56(3):225-34. doi: 10.1016/0027-5107(78)90189-6. de Serres FJ, Brockman HE, Hung CY, Overton LK. What do I do with my Chimeras after transfer? Multiple antimutagenesis mechanisms affect mutagenic activity and specificity of the base analog 6-N-hydroxylaminopurine in bacteria and yeast. Genet Mol Biol. This facility consists of an animal room and several injection rooms. University of Pennsylvania - ASLAP Current studies at the Center are focused on exploring the biology of germline stem cells, as well as male and female gametes: Professor of Swine Health and Reproduction sharing sensitive information, make sure youre on a federal . Personnel. Technical Director, Institute for Diabetes, Obesity and Metabolism For embryo freezing, we recommend transferring 10 females 6-8 weeks old. The Trustees of the University of Pennsylvania. Biochemical characterization of a novel hypoxanthine/xanthine dNTP pyrophosphatase from Methanococcus jannaschii. 1993 Oct;101 Suppl 3(Suppl 3):21-6. doi: 10.1289/ehp.93101s321. 1999 Jul;152(3):1065-77. doi: 10.1093/genetics/152.3.1065. Moved Permanently. Further, the core offers training in many of the above techniques on a fee-for-service basis. Phone: 215-898-0198 | Fax 215-898-5408, Trustees of the University of Pennsylvania, Citing the Diabetes Research Center (DRC) in Publications. Personnel - Penn Genetics A primary function of the core is to provide a centralized laboratory that will generate infection-free strains of mice carrying transgenes or targeted alterations of specific interest to individual projects in the DRC. The Transgenic Mouse Genome Editing Core has generated a wide range of transgenic and gene-targeted mouse lines that have proven to be powerful tools for the study of the molecular biology and physiology of Type I and Type II diabetes, obesity, and developmental and mechanistic bases for a wide variety of other disorders of endocrinology and met. Transgenic Mouse Genome Editing Core - Perelman School of Medicine at Email: epsteind@pennmedicine.upenn.edu, Tel: 215-898-6064 (office) or 215-573-3023, Tel: 215-573-3023; 215-746-6392 Advising@Penn. Transfer applicants must have completed a minimum of eight transferable college courses, including the following prerequisites: If you have a bachelors degree in a non-nursing discipline or are an RN Return Student (you have completed a diploma RN program or an associates degree RN program), you must apply to the Accelerated BSN Second Degree or BSN/MSN program. The Department of Genetics provides administrative support for several core facilities which in turn provide a variety of services to research laboratories across the School of Medicine and other affiliated institutions. The Transgenic Mouse Core located in the School of Veterinary Medicine is a state-of-the-art fee-for-service facility that offers a full line of embryological manipulation services, focused on, but not limited to, murine model systems. This site needs JavaScript to work properly. It is located in a barrier, pathogen-free small animal facility supervised by trained veterinarians. Penn A-Z | University of Pennsylvania An improved understanding of the basic processes governing germ cell and embryo development and of the biology of gametes (sperm and oocytes) and embryonic stem cells will enable us to improve reproductive efficiency, generate animal models of human and animal disease and help provide the knowledge base for regenerative medicine, as well as toward the treatment of infertility. We offer a 20% discount on your Injections if you choose to utilize our in house Es Cell derivation services. Advancing Women in Engineering. Mutational specificity of the base analogue, 2-aminopurine, in Escherichia coli. Elderly's intention to use technologies: A systematic - PubMed Persing DH, McGinty L, Adams CW, Fowler RG. vendor, received from a collaborator, custom-made by a core or created in the lab. Home Research Core Resources & Facilities Transgenic Mouse Core Services Our Services Generating Transgenic Mice Pronuclear DNA & CRISPR Injections Direct microinjection into the pronuclei of fertilized single-cell mouse embryos More Information Embryonic Stem Cell (ES-cell) Derivation and Injections The facility uses sterile food and water as well as autoclaved cages and bedding; all cages are of the microisolator type to limit the spread of colony infection. The tryptophan synthetase gene A series of mutants in E. coli has been used to examine the mutational specificity of over 80 purine base analogues. The Transgenic and Chimeric Mouse Facility provides a centralized service to efficiently produce infection-free transgenic, chimeric, and genome-edited strains of mice. From those 2 males we can freeze down 20 sperm straws (10 straws per male). iLab Organizer - Agilent